Immunoexpression of testis-specific histone 2B in human spermatozoa and testis tissue
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چکیده
منابع مشابه
P-116: Absence of JMJD1A, A Testis- Specific Histone Demethylase, in Tissue Samples of TESE Negative Men
Background During mammalian spermatogenesis unique and dynamic epigenetic events occur leading to chromatin condensation. Through these events, histone demethylases such as JMJD1A play important roles in compaction of sperm chromatin, due to regulation of histone methylation dynamics and alteration of chromatin structure. As �histone methylation� is one of the best-characterized modifications i...
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BACKGROUND Bull fertility is the degree of sperm's ability to fertilize and activate the egg and support embryo development, and this is critical for herd reproductive performance. We used the bull as a unique model organism for the study of male fertility because cattle genetics and physiology is similar to those of other mammals including humans. Moreover, reliable fertility data along with w...
متن کاملTestis/sperm-specific histone 2B in the sperm of donors and subfertile patients: variability and relation to chromatin packaging.
BACKGROUND The compaction of human sperm chromatin is the result of replacement of approximately 85% of histones with protamines. Germ-line testis/sperm-specific histone 2B (TSH2B) has been detected in only approximately 30% of mature spermatozoa. Its level in the semen of subfertile patients varies; its function is unknown. We evaluated TSH2B in the sperm samples of 23 donors and 49 subfertile...
متن کاملStructural basis of instability of the nucleosome containing a testis-specific histone variant, human H3T.
A histone H3 variant, H3T, is highly expressed in the testis, suggesting that it may play an important role in the chromatin reorganization required for meiosis and/or spermatogenesis. In the present study, we found that the nucleosome containing human H3T is significantly unstable both in vitro and in vivo, as compared to the conventional nucleosome containing H3.1. The crystal structure of th...
متن کاملStructure of human nucleosome containing the testis-specific histone variant TSH2B
The human histone H2B variant TSH2B is highly expressed in testis and may function in the chromatin transition during spermatogenesis. In the present study, the crystal structure of the human testis-specific nucleosome containing TSH2B was determined at 2.8 Å resolution. A local structural difference between TSH2B and canonical H2B in nucleosomes was detected around the TSH2B-specific amino-aci...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 1998
ISSN: 0268-1161,1460-2350
DOI: 10.1093/humrep/13.6.1559